What is the surface area of a 6 well plate?
What is the surface area of a 6 well plate?
Useful information for various sizes of cell culture dishes and flasks
| Catalog No. | Surface area (cm2) | |
|---|---|---|
| Dishes | ||
| 6-well | 140675 | 9.6 |
| 12-well | 150628 | 3.5 |
| 24-well | 142475 | 1.9 |
What is the area of a well in a 96 well plate?
96-Well Plates
| Cat.No. | Brand | Growth Area |
|---|---|---|
| 83-3596 | Corning® | 0.32cm2 |
| 83-3997 | Corning® | 0.32cm2 |
| 83-3598 | Corning® | 0.32cm2 |
| 83-3599 | Corning® | 0.32cm2 |
How big is a well in a 6 well plate?
Growth Area of Tissue Culture Plates and Dishes
| Growth Area (cm2) | Media Volume (ml) | |
|---|---|---|
| 245 mm (square) | 500 | 100-150 |
| Dishes | ||
| 6 well | 9.5 | 1.9-2.0 |
| 12 well | 3.8 | 0.76-1.14 |
How many cells are in a well of 96 well plate?
Typically, 20,000 – 75,000 cells are seeded per well in Oris™ and Oris™ Pro 96-well plates, and 2,500 – 10,000 per well in Oris™ Pro 384-well plates.
How much media do I need for a 96-well plate?
For any cell type you can use a minimum of 100ul to a maximum of 200ul culture medium per well in a 96-well plate.
How do you figure surface area?
The total surface area is calculated by adding all the areas on the surface: the areas of the base, top, and lateral surfaces (sides) of the object. This is done using different area formulas and measured in square units. Volume is the amount of space that a three dimensional object takes up.
How much media should be in a 6-well plate?
I usually add 1-2 mL of media in each well in the 6-well plate.
How much does it cost to seed a 6-well plate?
2D cultures
| Culture plate format | Number of viable cells per well/flask | Volume per well/flask |
|---|---|---|
| 6-well plate | 2 x 106 | 2 ml |
| 12-well plate | 0.8 x 106 | 1 ml |
| 24-well plate | 0.48 x 106 | 0.5 ml |
| 48-well plate | 0.16 x 106 | 0.2 ml |
How much volume does a 96-well plate have?
CulturPlate Microplates
| Well format | 24-well | 96-well |
|---|---|---|
| Well volume | 2.39 mL | 400 µL |
| Recommended working volume | 0.5-2.39 mL | 80 µL-350 µL |
| Height (mm) | 18.70 | 14.60 |
| Length (mm) | 127.80 | 127.76 |
What are the dimensions of a 96-well plate?
The length of the 96-well standard microplate is 127.71 mm x the width 85.43 mm and the height is 14.10 mm (shown in fig….ANSI- /SLAS – Standard 1 to 4 – 2004.
| 96-Well Standard Plate Dimensions | |
|---|---|
| L | 127.71 mm |
| W | 85.43 mm |
| H | 14.10 mm |
| X1 | 14.36 mm |
How much does it cost to seed a 6 well plate?
How do you incubate a 96-well plate?
Pipette 25 µL protease-inhibitor-supplemented Cell Extraction Buffer into each well. Incubate the plate on ice for 30 minutes. Thoroughly mix the contents of each well by pipetting up and down 5–6 times. A multi-channel pipette is desirable for this application.
How much volume does a 96 well plate hold?
How much can a 96 well plate hold? Well Plate diameter: 6.8 mm; 96 well volume: 360 μL; Working volume: 75-250 μL;
What is the size of a 96-well microplate?
Corning 96-well Microplates Flat Bottom 6.4 0.32 3.2 x 104 360 100 – 200 Round Bottom 6.4 N/A** N/A** 330 100 – 200 V-Bottom 6.4 0.38 3.8 x 104 320 100 – 200 Half Area 4.5 0.16 1.6 x 104 190 50 – 100 Corning 384-well Microplates Standard 2.7 x 2.7* 0.056 5.6 x 103 112 25 – 50
What is the surface area of a Corning well?
**Because these wells are round, the surface area available for cell attachment is dependent on the medium volume used. Corning Multiple Well Plates Single Well Only Well Approx. Total Well Working Diameter Growth Average Volume Volume Plate (Bottom) (mm) Area (cm2) Cell Yield (mL) (mL) 6-well 34.8 9.5 9.5 x 105 16.8 1.9 – 2.9
What are 6 12 24 48 48 and 96 well plates?
6, 12, 24, 48 and 96-Well Plates and Microplates, Corning®. By Corning®. These clear polystyrene plates have nonreversible lids with condensation rings to reduce contamination. Selection of 6-, 12-, 24-, 48 or 96- well plates. They feature individual alphanumerical codes for well identification.